更新時間:2024-03-14
Kerafast抗體Anti-DNA-RNA Hybrid [S9.6] :Anti-DNA-RNA Hybrid [S9.6] Antibody recognizes RNA-DNA hybrids of various lengths.
特色:
可用于檢測 R 環
對 DNA-RNA 雜交體的高特異性和親和力
不與單鏈 DNA 或雙鏈 DNA 發生交叉反應
對于富含 AU 的雙鏈 RNA,觀察到了輕微的交叉反應(約 5 倍以下)。
長度為 8、10、15 和 23 個堿基對的雜交體顯示出高親和力結合
DNA-RNA 雜合體是真核細胞中的一種自然現象,這些雜合體的水平在具有高轉錄活性的位點增加,例如在轉錄起始、抑制和延伸期間。由于 RNA-DNA 雜合體會影響基因組的不穩定性,因此 S9.6 抗體是一種有用的試劑,可幫助研究在 DNA 復制或其他細胞過程中由這些雜合體形成的 R 環和損傷的后果。此外,S9.6 抗體可有效識別用于微陣列研究的 RNA-DNA 雜交。
This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.
Highlights:
* Useful in the detection of R-loops
* High specificity and affinity for DNA-RNA hybrids
* Does NOT cross-react with single-stranded DNA or double-stranded DNA
* Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.
* High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length
產品詳情:
Product Type: | Antibody |
Name: | Anti-DNA-RNA Hybrid [S9.6] |
Antigen: | S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen |
Isotype: | Rabbit IgG |
Fusion Tag(s): | Mouse Fab version contains His-tag |
Clone Name: | S9.6 |
Reactivity: | High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids |
Immunogen: | ΦX174 bacteriophage-derived synthetic DNA/RNA |
Purification Method: | Protein A/G |
Buffer: | ENHOO1: PBS, 0.05% (w/v) Sodium Azide Ab01137- : PBS with 0.02% Proclin 300 |
Tested Applications: | Dot Blot Analysis: 0.2 µg/mL. |
參考文獻:
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